P-160: Study of Association between Polymorphism in Estrogen Response Element (ERE) in Promoter Region of C3 Gene and Spontaneous Recurrent Abortion

Background: There are different etiological factors in spontaneous recurrent abortion which are one of the complications in pregnancy. Primary embryo development in uterine tube and oviduct are affected by different factors such as embryotrophic elements. ETF3 as a embryotrophic factor that contains a complex of complement 3 protein and its derivatives specially iC3b, causes embryo trophoblast growth and expression of genes involved in embryo development. There are different response elements in promoter region of C3 gene such as two estrogen response elements (ERE). Materials and Methods: In this research, we proposed that polymorphism in two ERE in promoter region of C3 gene can associate with Spontaneous Recurrent Abortion in early months of pregnancy. Peripheral blood samples of 40 women with Spontaneous Recurrent Abortion registered to infertility center and 30 fertile women as control group were collected. After DNA extraction and amplification of regions harboring ERE utilizing PCR with specific primers, SSCP method used for study of probable polymorphisms in this region. Results: Our studies showed no significant correlation between polymorphism in ERE of C3 gene promoter and desired symptoms. However, this study closed our opinion to an individual asymptomatic infertility. Conclusion: Study of effective factors on this promoter region can be lionized. Accurate selection of more cases can be helpful in improvement or rejection of this proposal. Likewise as a new proposal, we emphasize on association between polymorphism in this region with some of heredity infertility of women.